Journal: Journal of Functional Biomaterials

Article Title: Cytocompatibility Assessment of L-PBF-Manufactured Zinc–Silver–Copper Alloys for Customized Biodegradable Medical Implants

doi: 10.3390/jfb17030146

Figure Lengend Snippet: ( A ) Representative images of the direct cytocompatibility contact test with SAOS-2 cells are presented. The top row ( a ) shows the tested sample plates (black) at the top of the image, with cells growing in direct contact on the bottom of the six-well plate (five times magnification). ( b ) The cell density at a 12 mm distance from the test plates (ten times magnification). ( B , D ) Three different extract concentrations (undiluted, diluted 1:3 and diluted 1:10) from the three L-PBF-manufactured Zn alloys (ZnAgCu, ZnAgCuMn, and ZnAgCuTi), rolled Zn, Cu, and Ti controls were tested, and the proliferation of L929 ( B ) and SAOS-2 ( D ) cells was determined after 24 h. ( C , E ) Live/dead staining of L929 ( C ) and SAOS-2 ( E ) cells growing directly on top of the samples is shown. Red and yellow staining indicate dead or dying cells, respectively, while living cells appear green. Each test was carried out four times with four replicates per sample. Shown are the mean values with their corresponding standard deviations. All results were normalized to control cells grown in cell culture medium without extract (proliferation equals 100%). To calculate statistical significance, a one-way ANOVA followed by a Tukey test was carried out (** p < 0.01 and **** p < 0.0001).

Article Snippet: In addition, the human primary osteogenic sarcoma cell line SAOS-2 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH), cultured in McCoy’s 5A medium (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) supplemented with 15% FBS (Bio&SELL GmbH) and 1% L-glutamine (Thermo Fisher Scientific Inc.), was used.

Techniques: Staining, Control, Cell Culture

Journal: Journal of Functional Biomaterials

Article Title: Cytocompatibility Assessment of L-PBF-Manufactured Zinc–Silver–Copper Alloys for Customized Biodegradable Medical Implants

doi: 10.3390/jfb17030146

Figure Lengend Snippet: Time dependency of ion release and cytocompatibility of undiluted extracts tested for 24 h with SAOS-2 cells from untreated ( A ) and polished ( B ) L-PBF-manufactured plates. Extracts collected daily over a period of 10 days. ( C ) OM of untreated and polished plates before and after 10 days of immersion. The mean proliferation values with the corresponding standard deviation of independent experiments ( n = 4) are plotted graphically. All results were normalized to the control cells grown in cell culture medium without extract (=100%). Significance was determined using one-way ANOVA followed by a Tukey test (* p < 0.05; ** p < 0.01; *** p < 0.001; and **** p < 0.0001). ( D , E ) ICP-OES ion concentration measurement. ( D ) Zn 2+ release of the different alloys into daily renewed McCoy’s medium from day 1 to day 10. The extracts were taken from untreated and freshly polished samples. The mean values with the corresponding standard deviations of five independent experiments are plotted. ( E ) Ion concentrations of Zn 2+ , Ag + , Cu 2+ , and Mn 2+ release of ZnAgCuMn into the daily changed McCoy’s medium ( n = 5).

Article Snippet: In addition, the human primary osteogenic sarcoma cell line SAOS-2 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH), cultured in McCoy’s 5A medium (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) supplemented with 15% FBS (Bio&SELL GmbH) and 1% L-glutamine (Thermo Fisher Scientific Inc.), was used.

Techniques: Standard Deviation, Control, Cell Culture, Concentration Assay

Journal: Journal of Functional Biomaterials

Article Title: Cytocompatibility Assessment of L-PBF-Manufactured Zinc–Silver–Copper Alloys for Customized Biodegradable Medical Implants

doi: 10.3390/jfb17030146

Figure Lengend Snippet: Cytocompatibility extract test with SAOS-2 cells cultivated for 24 h in extracts from untreated, new polished and aged polished samples. The mean proliferation values with the corresponding standard deviation of independent experiments ( n = 8) are plotted graphically. All results were normalized to the control cells grown in cell culture medium without extract (=100%). Significance was determined using one-way ANOVA followed by a Tukey test (* p < 0.05; ** p < 0.01).

Article Snippet: In addition, the human primary osteogenic sarcoma cell line SAOS-2 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH), cultured in McCoy’s 5A medium (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) supplemented with 15% FBS (Bio&SELL GmbH) and 1% L-glutamine (Thermo Fisher Scientific Inc.), was used.

Techniques: Standard Deviation, Control, Cell Culture

Journal: Journal of Cell Communication and Signaling

Article Title: Phosphoinositide-specific phospholipase C isoforms are conveyed by osteosarcoma-derived extracellular vesicles

doi: 10.1007/s12079-020-00571-6

Figure Lengend Snippet: Localization of PI-PLC isoforms into EVs

Article Snippet: Human osteosarcoma cell line SaOS2 (HTL01001), were purchased from Banca Biologica and Cell Factory (IRCCS Azienda Ospedaliera Universitaria San Martino-IST, Genova, Italy), while osteosarcoma cell line 143B (CRL-8303) and Hs-888.T (CRL-7622) was purchased from ATCC (Manassas, VA, USA).

Techniques:

Journal: Frontiers in Oncology

Article Title: Molecular features and predictive models identify the most lethal subtype and a therapeutic target for osteosarcoma

doi: 10.3389/fonc.2023.1111570

Figure Lengend Snippet: SQLE knockdown produced significant inhibition on cancer-related phenotypes of U2OS cells in vitro . (A) Quantitative PCR (Q−PCR) with mRNA expression confirmed the knockdown of SQLE after 24h siRNA transfection. (B) Western blot analyses with protein expression confirmed the knockdown of SQLE after 48h siRNA transfection. The β-actin was treated as the loading control. (C) Cell proliferation was suppressed by SQLE knockdown. (D) Cell colony formation ability was reduced by SQLE knockdown. (E) Cell migration was inhibited by SQLE knockdown. The scale bar represented 100 μm. (F) and (G) indicated the quantification of positive signals from (D, E) , respectively. (H) The knockdown of SQLE significantly reduced the intracellular cholesterol. Error bars represented ± SD of three biological replicates. **P < 0.01, ***P < 0.001 by Student t-test.

Article Snippet: The U2OS and Saos-2, human osteosarcoma cell lines, were obtained from the American Type Culture Collection (ATCC, VA, USA).

Techniques: Produced, Inhibition, In Vitro, Real-time Polymerase Chain Reaction, Expressing, Transfection, Western Blot, Migration

Journal: Frontiers in Oncology

Article Title: Molecular features and predictive models identify the most lethal subtype and a therapeutic target for osteosarcoma

doi: 10.3389/fonc.2023.1111570

Figure Lengend Snippet: Terbinafine treatment impaired the growth and migration of U2OS cells in vitro . (A) Different gradient concentrations of terbinafine were added to the U2OS cells. As the dosage of terbinafine increased, the viability of the cells decreased. (B) The U2OS cells were respectively treated with 25 μM and 50 μM terbinafine. Cell colony formation ability was found reduced with the increasing dosage of terbinafine. (C) The number of migrating cells decreased obviously with the increase in drug concentration. Scale bars, 100 μm. (D, E) indicated the quantification of positive signals from (B, C) , respectively. (F) The terbinafine treatment significantly reduced the intracellular cholesterol. Error bars represent mean ± SD; statistical analysis was performed using the Student t-test. **P < 0.01, ***P < 0.001.

Article Snippet: The U2OS and Saos-2, human osteosarcoma cell lines, were obtained from the American Type Culture Collection (ATCC, VA, USA).

Techniques: Migration, In Vitro, Concentration Assay